Scanning electron microscopy analysis of RinsEndo system and conventional irrigation for debris removal.

A new device for irrigation, which presents hydrodynamic activation based on the pressure-suction technology, has recently been introduced to the market: the RinsEndo system. This study compared the efficacy of the RinsEndo system and conventional (manual-dynamic) irrigation in the removal of debris from the root canal walls, using scanning electron microscopy (SEM). Twenty mandibular premolars with completely formed roots were selected and randomly divided into group 1 (irrigation with the RinsEndo system) and group 2 (conventional irrigation). The canals were irrigated with 1 mL of saline at each change of instrument. Instrumentation started with a #15 K file and continued up to a #40 K file, which was standardized as the working length instrument. Then, the teeth were sectioned in buccolingual direction and the halves were sputter-coated with gold and examined by SEM. The apical, middle and cervical root canal thirds were evaluated, and the results were analyzed statistically by the Mann-Whitney test for comparison between methods, Kruskal-Wallis test for comparison among thirds, and Miller test for individual comparisons. A significance level of 5% was set for all analyses. The results did not show significant differences (p>0.05) between methods at each third and among thirds for each technique analyzed individually. In conclusion, there was no difference in the cleaning ability of the RinsEndo system and conventional irrigation.

Scanning electron microscopy analysis of RinsEndo system and conventional irrigation for debris removal

A new device for irrigation, which presents hydrodynamic activation based on the pressure-suction technology, has recently been introduced to the market: the RinsEndo system. This study compared the efficacy of the RinsEndo system and conventional (manual-dynamic) irrigation in the removal of debris from the root canal walls, using scanning electron microscopy (SEM). Twenty mandibular premolars with completely formed roots were selected and randomly divided into group 1 (irrigation with the RinsEndo system) and group 2 (conventional irrigation). The canals were irrigated with 1 mL of saline at each change of instrument. Instrumentation started with a #15 K file and continued up to a #40 K file, which was standardized as the working length instrument. Then, the teeth were sectioned in buccolingual direction and the halves were sputter-coated with gold and examined by SEM. The apical, middle and cervical root canal thirds were evaluated, and the results were analyzed statistically by the Mann-Whitney test for comparison between methods, Kruskal-Wallis test for comparison among thirds, and Miller test for individual comparisons. A significance level of 5 percent was set for all analyses. The results did not show significant differences (p>0.05) between methods at each third and among thirds for each technique analyzed individually. In conclusion, there was no difference in the cleaning ability of the RinsEndo system and conventional irrigation.

Um novo método para irrigação, que apresenta ativação hidrodinâmica baseado na tecnologia de pressão e aspiração, foi recentemente introduzido no mercado: o sistema RinsEndo. O presente estudo comparou a eficácia do sistema RinsEndo e irrigação convencional(manual-dinâmica) na remoção de debris do canal radicular, empregando-se microscopia eletrônica de varredura (MEV). Vinte pré-molares inferiores com raiz completamente formada foram selecionados e divididos aleatoriamente em grupo 1 (sistema RinsEndo) e grupo 2 (irrigação convencional). Os canais foram irrigados com 1 mL de solução salina a cada troca de instrumento. A instrumentação foi iniciada com uma lima tipo K #15 dilatando-se até lima tipo K #40, no comprimento de trabalho. Posteriormente os dentes foram clivados no sentido vestíbulo-lingual e as hemisecções foram metalizadas com ouro e analisadas em MEV. Os terços cervical, médio e apical foram avaliados, e os resultados foram analisados estatisticamente pelo teste de Mann-Whitney para comparação entre métodos, e o teste Kruskal-Wallis para comparação entre terços e o teste de Miller para os confrontos individuais. Foi empregado o nível de significância de 5 por cento para todas as análises. Os resultados não mostraram diferença estatisticamente significante (p>0,05) entre métodos em cada terço, nem entre terços para cada técnica analisada individualmente. Concluiu-se que não houve diferença na capacidade de limpeza do sistema RinsEndo e da irrigação convencional.

Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno: estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico / Comparative analysis of the tissue response to the implantation of polyethylene tubes, dentin tubes and collagen capsules: a microscopic study in subcutaneous tissue of rats. Evaluation of a methodology model

Para se avaliar a citoxicidade de materiais endodônticos, uma das metodologias utilizadas é a implantação de amostras dos materiais no tecido conjuntivo de pequenos animais, e para essa implantação são utilizados acondicionadores. O propósito deste estudo foi comparar as reações teciduais de alguns acondicionadores utilizados neste tipo de pesquisa, propondo desta maneira uma melhor metodologia para ser utilizada em trabalhos futuros. Foram implantados no tecido subcutâneo de 54 ratos, tubos de polietileno, bastões de dentina e cápsulas de colágeno, formando 3 grupos que permaneceram com os implantes pelos períodos de 15, 30 e 60 dias. Os espécimes dos grupos I e II foram analisados pela microscopia óptica de modo descritivo e morfométrico, considerando 5 critérios morfológicos: fibras colágenas, fibroblastos, vasos sanguíneos, células inflamatórias e outros componentes. O grupo III foi submetido, apenas, à análise descritiva. Os valores médios encontrados nos grupos I e II foram submetidos ao Teste t de Student para comparação entre os grupos nos períodos experimentais. O Teste ANOVA foi aplicado para comparar os períodos, nos grupos experimentais, e os valores significantes foram submetidos ao teste de Tukey. Os resultados obtidos revelaram que os tubos de polietileno e bastões de dentina induziram reações teciduais semelhantes, demonstrando, aos 60 dias, um comportamento de reparo. As cápsulas de colágeno foram reabsorvidas e houve recomposição morfológica do tecido subcutâneo.

With the purpose of evaluating the citotoxicity of some endodontic materials, their implantation in subcutaneous tissue of small animals is performed, with the aid of containers, which are used to carry the testing materials. The aim of this study was to compare the subcutaneous tissue response related to the implantation of polyethylene tubes, dentin tubes and collagen capsules usually used as carrier materials in this kind of methodology. 54 rats were divided in 3 groups: polyethylene tubes (Group I), dentin tubes (Group II) and collagen capsules (Group III). After 15, 30 and 60 days of implantation, the animals were killed and the specimens of group I and II were prepared for descriptive and morphometric analysis considering: collagen fibers, fibroblasts, vessels, inflammatory cells and other components. The group III (collagen capsules) was only evaluated through descriptive analyses. The average values of group I and II were submitted to T student test for comparison between the groups in the experimental periods. ANOVA-Tukey test was used to show a difference in the tissue response in the different periods to each material. The results showed similar tissue reaction between polyethylene and dentin tubes, showing organization of the tissue at 60 days. The collagen capsules were resorbed showing morphological recomposition of the subcutaneous tissue.